Research Area

Our research directions are metabolic engineering of industrial microorganisms such as Clostridia, Escherichia coli, Bacillus subtilis and yeast; protein engineering of industrial enzymes; development and application of new technologies for engineering genes, proteins, pathways and genomes. The goal for these work is to develop and optimize genetically-based bioproducts and bioprocesses.

Team Description

The team’s research focuses on metabolic engineering and genomic editing in bacteria and yeast. Our research goal is the development of synthetic biology-based biocatalysts for industrial use by providing new enabling technologies and novel designs. Our scientific objectives include 1) a more efficient enzyme production protocol and facility for synthetic chemistry, 2) more efficient microbial genome editing tools for faster construction of designer cells, and 3) building genetically defined cells to understand the mechanism behind the efficient catalytic performance of evolved biocatalysts.

Team Members

Junjie Yang
Huiqi He
Yiwen Zhang

Team Show


1、Bingbing Sun, Haiyan Liu , Yu Jiang, Lei Shao, Sheng Yang*, Daijie Chen*(2020) New Mutations Involved in Colistin Resistance in Acinetobacter baumannii. mSphere. 5(2):e00895-19

2、Qi Li, Meixian Wu, Zhiqiang Wen, Yuan Jiang, Xin Wang, Yawei Zhao, Jinle Liu, Junjie Yang, Yu Jiang, Sheng Yang*(2020) Optimization of n‑butanol synthesis in Lactobacillus brevis via the functional expression of thl, hbd, crt and te.Journal of Industrial Microbiology & Biotechnology. doi:10.1007/s10295-020-02331-2

3、Xiaoman Sun, Zixu Zhang, Lingru Wang, Jinggang Wang, Yan Liang, Haifeng Yang, Rongsheng Tao, Yu Jiang, Junjie Yang, Sheng Yang* (2020) Downregulation of T7 RNA polymerase transcription enhances pET‐based recombinant protein production in Escherichia coli BL21 (DE3) by suppressing autolysis. Biotechnology and Bioengineering. doi: 10.1002/bit.27558

4、Jiao Zhang, Fenghui Qian, Feng Dong, Qingzhuo Wang, Junjie Yang, Yu Jiang*, Sheng Yang* (2020) De Novo Engineering of Corynebacterium glutamicum for l-Proline Production. ACS Synth Biol. doi: 10.1021/acssynbio.0c00249

5、Yiwen Zhang, Xiaoman Sun, Qingzhuo Wang, Jiaqi Xu, Feng Dong, Siqi Yang, Jiawei Yang, Zixu Zhang, Yuan Qian, Jun Chen, Jiao Zhang, Yingmiao Liu, Rongsheng Tao, Yu Jiang, Junjie Yang*, Sheng Yang* (2020) Multicopy chromosomal integration using CRISPR-associated transposases. ACS Synth Biol. doi: 10.1021/acssynbio.0c00073

6、Jinle Liu, Yu Jiang, Jun Chen, Junjie Yang, Weihong Jiang, Wei Zhuang, Hanjie Ying, Sheng Yang* (2020) Metabolic engineering and adaptive evolution of Clostridium beijerinckii to increase solvents production from corn stover hydrolysate. J Agric Food Chem. doi: 10.1021/acs.jafc.0c03048

7、Zhiqiang Wen, Qi Li, Jinle Liu, Mingjie Jin*, Sheng Yang* (2020) Consolidated bioprocessing for butanol production of cellulolytic Clostridia: development and optimization. Microb Biotechnol. 13(2):410-422

8、Zhiqiang Wen, Minrui Lu, Rodrigo Ledesma-Amaro, Qi Li, Mingjie Jin*, Sheng Yang* (2020) TargeTron Technology Applicable in Solventogenic Clostridia: Revisiting 12 Years' Advances. Biotechnology journal. 15(1):e1900284

9、Zhiqiang Wen, Rodrigo Ledesma-Amaro, Minrui Lu, Yu Jiang, Shuliang Gao, Mingjie Jin*, Sheng Yang* (2020) Combined evolutionary engineering and genetic manipulation improve low pH tolerance and butanol production in a synthetic microbial Clostridium community. Biotechnology and Bioengineering. Doi: 10.1002/bit.27333

10、Zhiqiang Wen, Rodrigo Ledesma-Amaro, Minrui Lu, Mingjie Jin*, Sheng Yang* (2020) Metabolic Engineering of Clostridium cellulovorans to Improve Butanol Production by Consolidated Bioprocessing. ACS synthetic biology.Doi: 10.1021/acssynbio.9b00331

11、Qi Li, Francois M. Seys, Nigel P. Minton, Junjie Yang, Yu Jiang, Weihong Jiang, Sheng Yang*(2019)CRISPR-Cas9(D10A) nickase-assisted base editing in solvent producer Clostridium beijerinckii. Biotechnol Bioeng. Doi: 10.1002/bit.26949

12、He Huang, Xin Song, Sheng Yang*(2019) Development of a RecE/T-Assisted CRISPR-Cas9 Toolbox for Lactobacillus. Biotechnology journal. doi: 10.1002/biot.201800690.

13、Zhiqiang Wen, Ledesma-Amaro, Rodrigo; Jianping Lin*, Yu Jiang, Sheng Yang*(2019)Improved n-butanol production from Clostridium cellulovorans by integrated metabolic and evolutionary engineering. Appl Environ Microbiol. Doi: 10.1128/AEM.02560-18

14、Xinyun Jing, Bingran Xie, Longxian Chen, Niubing Zhang, Yiyi Jiang, Hang Qin, Hongbing Wang, Pei Hao*, Sheng Yang* , Xuan Li*(2018)Implementation of the CRISPR-Cas13a system in fission yeast and its repurposing for precise RNA editing. Nucleic Acids Res. Doi :10.1093/nar/gky433

15、Bingbing Sun, Junjie Yang, Sheng Yang*, Richard D. Ye*, Daijie Chen,*; Yu Jiang*(2018)A CRISPR-Cpf1-Assisted Non-Homologous End Joining Genome Editing System of Mycobacterium smegmatis. Biotechnology journal . 13 (9) :1700588

16、Xin Wang, Junjie Yang, Sheng Yang*, Yu Jiang*(2018)Unraveling the genetic basis of fast L-arabinose consumption on top of recombinant xylose-fermenting Saccharomyces cerevisiae. Biotechnol Bioeng. Doi:10.1002/bit.26827

17、Jun Sun, Qingzhuo Wang, Yu Jiang, Zhiqiang Wen, Lirong Yang, Jianping Wu* and Sheng Yang*(2018)Genome editing and transcriptional repression in Pseudomonas putida KT2440 via the type II CRISPR system. Microb Cell Fact. 17:41

18、Xin Song, He Huang, Zhi-Qiang Xiong, Lianzhong Ai*, Sheng Yang* (2017) CRISPR-Cas9D10A Nickase-Assisted Genome Editing in Lactobacillus casei. Appl. Environ. Microbiol. doi: 10.1128/AEM.01259-17

19、Qi Liu, Yu Jiang, Lei Shao, Ping Yang, Bingbing Sun,Sheng Yang*, and Daijie Chen* (2017) CRISPR/Cas9-based efficient genome editing in Staphylococcus aureus. Acta Biochim Biophys Sin. doi: 10.1093/abbs/gmx074

20、Xiaoshu Xu, Chao Wang, Jun Chen, and Sheng Yang* (2017)Streptomyces virginiae PPDC is a new type of phenylpyruvate decarboxylase composed of two subunits. ACS Chemical Biology 2017; doi:10.1021/acschembio.7b00307

21、Yu Jiang, Fenghui Qian, Junjie Yang, Yingmiao Liu, Feng Dong, Chongmao Xu, Bingbing Sun, Biao Chen, Xiaoshu Xu, Yan Li, Renxiao Wang, Sheng Yang* (2017) CRISPR-Cpf1 -assisted genome editing of Corynebacterium glutamicum. Nature Communications.doi:10.1038/ncomms15179 ESI高被引

22、Shuliang Gao, Yangyang Tong, Li Zhu, Ge Mei, Zhang Yian, Daijie Chen, Yu Jiang, Sheng Yang* (2017) Iterative integration of multiple-copy pathway genes in Yarrowia lipolytica for heterologous β-carotene production. Metabolic Engineering. 41:192-201

23、Zhiqiang Wen, Nigel P. Minton, Ying Zhang, Qi Li, Jinle Liu, c, Yu Jiang, Sheng Yang*(2017)Enhanced solvent production by metabolic engineering of a twin-clostridial consortium. Metabolic Engineering.39:38-48

24、Junjie Yang and Sheng Yang* (2016)Comparative analysis of Corynebacterium glutamicum genomes: a new perspective for the industrial production of amino acids. BMC Genomics . 18(1):940

25、Yu Jiang, Rongsheng Tao, Zhengquan Shen, Liangdong Sun, Fuyun Zhu, Sheng Yang*(2016)Enzymatic Production of Glutathione by Bifunctional γ-Glutamylcysteine Synthetase/Glutathione Synthetase Coupled with In Vitro Acetate Kinase-Based ATP Generation. Appl Biochem Biotechnol.180(7) 1446-1455

26、Shuliang Gao, Yangyang Tong, Zhiqiang Wen, Li Zhu, Mei Ge, Daijie Chen*, Yu Jiang*, Sheng Yang* (2016) Multiplex gene editing of the Yarrowia lipolytica genome using the CRISPR-Cas9 system. Journal of Industrial Microbiology & Biotechnology . 43(8)1085–1093

27、Yu Jiang Rongsheng Tao , Sheng Yang* (2016) Tailor-made biocatalysts enzymes for the fine chemical industry in China. Biotechnol Journal. 11,1121–1123

28、Qi Li, Jun Chen, Nigel P. Minton, Ying Zhang, Zhiqiang Wen, Jinle Liu, Haifeng Yang, Zhe Zeng, Xiaodan Ren, Junjie Yang, Yang Gu, Weihong Jiang, Yu Jiang, Sheng Yang* (2016) CRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii. Biotechnology Journal. 11(7):961-72

29、Chen Yang, Xiang Gao, Yu Jiang, Bingbing Sun, Fang Gao, Sheng Yang*(2016)Synergy between methylerythritol phosphate pathway and mevalonate pathway for isoprene production in Escherichia coli. Metabolic Engineering, 2016, 37:79–91

30、Xiaoshu Xu, Jun Chen, Qingzhuo Wang, Chunlan Duan, Yan Li, Renxiao Wang,Sheng Yang*(2016) Mutagenesis of Key Residues in the Binding Center of l-Aspartate-b-Semialdehyde Dehydrogenase from Escherichia coli Enhances Utilization of the Cofactor NAD(H), ChemBioChem.17:56-64

31、Yu Jiang, Biao Chen, Chunlan Duan, Bingbing Sun, Junjie Yang, and Sheng Yang* (2015)Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System. Appl. Environ. Microbiol. 81:2506-14 ESI高被引

32、Ning Zhang, Lijun Shao, Yu Jiang, Yang Gu, Qi Li, Jinle Liu, Weihong Jiang, Sheng Yang* (2015) I-SceI-mediated scarless gene modification via allelic exchange in Clostridium. Journal Microbial Methods. 108:49-60

33、Yu Jiang, Jinle Liu, Yunliu Yang, Weihong Jiang, Sheng Yang* (2014) Current status and prospects of industrial bio-production of n-butanol in China, Biotechnology Advances. 33:1493-1501

34、Junjie Yang, Bingbing Sun, He Huang, Yu Jiang, Liuyang Diao, Biao Chen, Chongmao Xua,Xin Wang, Jinle Liu, Weihong Jiang, Sheng Yang* (2014) High-efficiency Scarless Genetic Modification in Escherichia coli Using lambda-Red Recombination and I-SceI Cleavage. Appl. Environ. Microbiol. 80(13):3826-34

35、Rongsheng Tao, Yu Jiang, Fuyun Zhu, Sheng Yang* (2014) A one-pot system for production ofL-2-aminobutyric acid from L-threonine by L-threonine deaminase and a NADH regeneration system based on L-leucine dehydrogenase and formate dehydrogenase. Biotechnology Letters 36(4):835-41

36、Liuyang Diao, Yingmiao Liu, Fenghui Qian, Junjie Yang, Yu Jiang, Sheng Yang* (2013)Construction of fast xylose-fermenting yeast based on industrial ethanol-producing diploid Saccharomyces cerevisiaeby rational design and adaptive evolution. BMC Biotechnology 13:110

37、Zhilin Li, Han Xiao, Weihong Jiang, Yu Jiang*, Sheng Yang* (2013) Improvement of butanol production from xylose mother liquor by engineering xylose metabolic pathway in Clostridium acetobutylicum EA 2018. Applied Biochemistry and Biotechnology. 171(3):555-68

38、Han Xiao, Zhilin Li, Yu Jiang, Yunliu Yang, Weihong Jiang, Yang Gu*, Sheng Yang* (2012) Metabolic engineering of D-xylose pathway in Clostridium beijerinckii to optimize solvent production from xylose mother liquid. Metabolic Engineering 14: 565-578

39、Han Xiao, Yang Gu, Yuanyuan Ning, Yunliu Yang, W.J. Mitchell, Weihong Jiang*, Sheng Yang* (2011) Confirmation and elimination of xylose-metabolic bottlenecks in glucose-PTS-deficient Clostridium acetobutylicum to realize simultaneous utilization of glucose, xylose and arabinose. Appl. Environ. Microbiol 77:7886-7895

40、Zhoutong Sun, Yuanyuan Ning, Lixia Liu, Yingmiao Liu, Bingbing Sun, Weihong Jiang, Chen Yang and Sheng Yang* (2011) Metabolic engineering of the L-phenylalanine pathway in Escherichia coli for the production of S- or R-mandelic acid. Microbial Cell Factories. 10:71 Highly Accessed

41、Li Zhu, Rongsheng Tao, Yi Wang, Yu Jiang, Xin Lin, Yunliu Yang, Huabao Zheng, Weihong Jiang, Sheng Yang* (2011) Removal of L-alanine from the production of L-2-aminobutyric acid by introduction of alanine racemase and D-amino acid oxidase. Appl Microbiol Biotechnol. 90:903-910

42、Jian LI, Jingbo ZHAO, Meng ZHAO, Yunliu YANG, Weihong JIANG, Sheng YANG* (2010)Screening and Characterization of Butanol Tolerant Microorganisms. Letters in Applied Microbiology 50: 373-9

43、Yu JIANG, Chongmao XU, Feng DONG, Yunliu YANG, Weihong JIANG*, Sheng YANG* (2009) Disruption of the acetoacetate decarboxylase gene in solvent-producing Clostridium acetobutylicum increases the butanol ratio. Metabolic Engineering 11: 284-91

44、Junjie YANG, Weihong JIANG, Sheng YANG* (2009). mazF as a counter-selectable marker for unmarked genetic modification of Pichia pastoris. FEMS Yeast Research. 9(4): 600-9

45、Hong YU, Jian LI, Dalong ZHANG, Yunliu YANG, Weihong JIANG, Sheng YANG* (2009). Improving the thermostability of N-carbamyl-D-amino acid amidohydrolase by error-prone PCR. Appl. Microbiol. Biotechnol. 82(2): 279-85

46、Lijun SHAO, Shiyuan HU, Yi YANG, Yang GU, Jun CHEN, Yunliu YANG, Weihong JIANG*, Sheng YANG* (2007) Targeted gene disruption by use of a group II intron (targetron) vector in Clostridium acetobutylicum. Cell Research. 17: 963-5