This material may be covered by one or more patents, trademarks and/or copy rights owned or controlled by Depositors or any third parties. This material is available to academic and nonprofit organizations for research use only. Please contact MolecularCloud at email@example.com and the Depositor directly if you are from industrial institute or attempt for profit application. This material is not intended to be used therapeutic or diagnostic purposed in humans or animals.
L. Yin, L. A. Maddison, M. Li, N. Kara, M. C. LaFave, G. K. Varshney, S. M. Burgess, J. G. Patton, and W. Chen, 'Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and Sgrnas', Genetics, 200 (2015), 431-41.
Plasmid Size (bp)
* If this plasmid is used for your research, please acknowledge the Depositor and cite this article. In addition, please include MolecularCloud Cat. No. in the Materials and Methods section of your future publications.
The plasmid is used for 2019-nCoV(SARS-CoV-2) surface glycoprotein expression (Codon Optimized for Mouse expression system). The surface glycoprotein is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.