Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 emerged as a substantial tool due to its simplicity in use, less cost and extraordinary efficiency than the conventional gene-editing tools.However, potential off-target activities are crucial shortcomings in ...Learn More


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Cancer appears from the own cells of an organism and creates a tumor microenvironment with immune evasion and immunosuppression signals capable of avoiding the immune response [1]. Immunotherapy is a medical strategy applied to the treatment of cancer based on harnessing the ...Learn More


Currently, there are several methods for delivering CRISPR systems in vivo and in vitro. Both delivery vectors and physical methods are widely implemented for the efficient delivery of CRISPR/Cas9-mediated genome editing. Delivery vectors such as viral and non-viral vectors can ...Learn More


the interview of Dr. Changhao Bi

Using CRISPR Cas9 gene editing two types of gene edits can be made, namely knockouts and knock-ins. In a gene knockout experiment, a double-strand break is introduced by the Cas9 nuclease which leads to Non-homologous end joining of the DNA, and hence insertions or deletions of ...Learn More


The gRNA guides the CRIPR Cas9 system to the right genomic location for performing a double strand break.The targerting specificity of the CRISPR Cas9 system is determined by the 20 nucleotides at the 5' end of the gRNA. The gRNA base pairs to the complementary strand of the ...Learn More


Cloud Scientists
  • Beate Escher photo

    Beate Escher

    Helmholtz Centre for Environmental Research–UFZ

  • Dimitri Kullmann photo

    Dimitri Kullmann

    UCL Queen Squaree Institute of Neurology

  • Changhao Bi photo

    Changhao Bi

    Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences

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