LOCUS Exported 22259 bp SYN 21-Oct-2026 DEFINITION . ACCESSION . VERSION . KEYWORDS . SOURCE . ORGANISM . . COMMENT . FEATURES Location/Qualifiers promoter complement(39..716) /label=CaMV 35S promoter (enhanced) /note="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" CDS 2811..4526 /codon_start=1 /product="N-terminal portion of Streptococcus pyogenes Cas9 (Zetsche et al., 2015)" /label=Cas9(N) /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE" promoter 7317..7994 /label=CaMV 35S promoter (enhanced) /note="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" CDS 8948..9004 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 9734..9790 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" polyA_signal 11474..11648 /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" misc_feature complement(11726..11750) /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" CDS 12175..12969 /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" rep_origin 13056..13644 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature complement(13830..13970) /label=bom /note="basis of mobility region from pBR322" rep_origin complement(14314..14508) /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" CDS complement(16075..16704) /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" misc_feature complement(18004..18028) /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" terminator complement(18050..18302) /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" ORIGIN 1 ccatggagag atagatttgt agagagagac tggtgatttc agcgtgtcct ctccaaatga 61 aatgaacttc cttatataga ggaaggtctt gcgaaggata gtgggattgt gcgtcatccc 121 ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt ggaacgtctt 181 ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg 241 catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct 301 tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc 361 ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt 421 tgatattctt ggagtagacg agagtgtcgt gctccaccat gttatcacat caatccactt 481 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc 541 catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat 601 gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag 661 ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag 721 ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgggat 781 ccccggggtc gtgcccctct ctagagataa tgagcattgc atgtctaagt tataaaaaat 841 taccacatat tttttttgtc acacttgttt gaagtgcagt ttatctatct ttatacatat 901 atttaaactt tactctacga ataatataat ctatagtact acaataatat cagtgtttta 961 gagaatcata taaatgaaca gttagacatg gtctaaagga caattgagta ttttgacaac 1021 aggactctac agttttatct ttttagtgtg catgtgttct cctttttttt tgcaaatagc 1081 ttcacctata taatacttca tccattttat tagtacatcc atttagggtt tagggttaat 1141 ggtttttata gactaatttt tttagtacat ctattttatt ctattttagc ctctaaatta 1201 agaaaactaa aactctattt tagttttttt atttaataat ttagatataa aatagaataa 1261 aataaagtga ctaaaaatta aacaaatacc ctttaagaaa ttaaaaaaac taaggaaaca 1321 tttttcttgt ttcgagtaga taatgccagc ctgttaaacg ccgtcgacga gtctaacgga 1381 caccaaccag cgaaccagca gcgtcgcgtc gggccaagcg aagcagacgg cacggcatct 1441 ctgtcgctgc ctctggaccc ctctcgagag ttccgctcca ccgttggact tgctccgctg 1501 tcggcatcca gaaattgcgt ggcggagcgg cagacgtgag ccggcacggc aggcggcctc 1561 ctcctcctct cacggcaccg gcagctacgg gggattcctt tcccaccgct ccttcgcttt 1621 cccttcctcg cccgccgtaa taaatagaca ccccctccac accctctttc cccaacctcg 1681 tgttgttcgg agcgcacaca cacacaacca gatctccccc aaatccaccc gtcggcacct 1741 ccgcttcaag gtacgccgct cgtcctcccc cccccccctc tctaccttct ctagatcggc 1801 gttccggtcc atggttaggg cccggtagtt ctacttctgt tcatgtttgt gttagatccg 1861 tgtttgtgtt agatccgtgc tgctagcgtt cgtacacgga tgcgacctgt acgtcagaca 1921 cgttctgatt gctaacttgc cagtgtttct ctttggggaa tcctgggatg gctctagccg 1981 ttccgcagac gggatcgatt tcatgatttt ttttgtttcg ttgcataggg tttggtttgc 2041 ccttttcctt tatttcaata tatgccgtgc acttgtttgt cgggtcatct tttcatgctt 2101 ttttttgtct tggttgtgat gatgtggtct ggttgggcgg tcgttctaga tcggagtaga 2161 attctgtttc aaactacctg gtggatttat taattttgga tctgtatgtg tgtgccatac 2221 atattcatag ttacgaattg aagatgatgg atggaaatat cgatctagga taggtataca 2281 tgttgatgcg ggttttactg atgcatatac agagatgctt tttgttcgct tggttgtgat 2341 gatgtggtgt ggttgggcgg tcgttcattc gttctagatc ggagtagaat actgtttcaa 2401 actacctggt gtatttatta attttggaac tgtatgtgtg tgtcatacat cttcatagtt 2461 acgagtttaa gatggatgga aatatcgatc taggataggt atacatgttg atgtgggttt 2521 tactgatgca tatacatgat ggcatatgca gcatctattc atatgctcta accttgagta 2581 cctatctatt ataataaaca agtatgtttt ataattattt tgatcttgat atacttggat 2641 gatggcatat gcagcagcta tatgtggatt tttttagccc tgccttcata cgctatttat 2701 ttgcttggta ctgtttcttt tgtcgatgct caccctgttg tttggtgtta cttctgcaga 2761 tggctcctaa gaagaagcgg aaggttggta ttcacggggt gcctgcggct gacaagaagt 2821 actccatcgg cctcgacatc ggcaccaaca gcgtcggctg ggcggtgatc accgacgagt 2881 acaaggtccc gtccaagaag ttcaaggtcc tgggcaacac cgaccgccac tccatcaaga 2941 agaacctcat cggcgccctc ctcttcgact ccggcgagac ggcggaggcg acccgcctca 3001 agcgcaccgc ccgccgccgc tacacccgcc gcaagaaccg catctgctac ctccaggaga 3061 tcttctccaa cgagatggcg aaggtcgacg actccttctt ccaccgcctc gaggagtcct 3121 tcctcgtgga ggaggacaag aagcacgagc gccaccccat cttcggcaac atcgtcgacg 3181 aggtcgccta ccacgagaag taccccacta tctaccacct tcgtaagaag cttgttgact 3241 ctactgataa ggctgatctt cgtctcatct accttgctct cgctcacatg atcaagttcc 3301 gtggtcactt ccttatcgag ggtgacctta accctgataa ctccgacgtg gacaagctct 3361 tcatccagct cgtccagacc tacaaccagc tcttcgagga gaaccctatc aacgcttccg 3421 gtgtcgacgc taaggcgatc ctttccgcta ggctctccaa gtccaggcgt ctcgagaacc 3481 tcatcgccca gctccctggt gagaagaaga acggtctttt cggtaacctc atcgctctct 3541 ccctcggtct gacccctaac ttcaagtcca acttcgacct cgctgaggac gctaagcttc 3601 agctctccaa ggatacctac gacgatgatc tcgacaacct cctcgctcag attggagatc 3661 agtacgctga tctcttcctt gctgctaaga acctctccga tgctatcctc ctttcggata 3721 tccttagggt taacactgag atcactaagg ctcctctttc tgcttccatg atcaagcgct 3781 acgacgagca ccaccaggac ctcaccctcc tcaaggctct tgttcgtcag cagctccccg 3841 agaagtacaa ggagatcttc ttcgaccagt ccaagaacgg ctacgccggt tacattgacg 3901 gtggagctag ccaggaggag ttctacaagt tcatcaagcc aatccttgag aagatggatg 3961 gtactgagga gcttctcgtt aagcttaacc gtgaggacct ccttaggaag cagaggactt 4021 tcgataacgg ctctatccct caccagatcc accttggtga gcttcacgcc atccttcgta 4081 ggcaggagga cttctaccct ttcctcaagg acaaccgtga gaagatcgag aagatcctta 4141 ctttccgtat tccttactac gttggtcctc ttgctcgtgg taactcccgt ttcgcttgga 4201 tgactaggaa gtccgaggag actatcaccc cttggaactt cgaggaggtt gttgacaagg 4261 gtgcttccgc ccagtccttc atcgagcgca tgaccaactt cgacaagaac ctccccaacg 4321 agaaggtcct ccccaagcac tccctcctct acgagtactt cacggtctac aacgagctca 4381 ccaaggtcaa gtacgtcacc gagggtatgc gcaagcctgc cttcctctcc ggcgagcaga 4441 agaaggctat cgttgacctc ctcttcaaga ccaaccgcaa ggtcaccgtc aagcagctca 4501 aggaggacta cttcaagaag atcgagtgct tcgactccgt cgagatcagc ggcgttgagg 4561 accgtttcaa cgcttctctc ggtacctacc acgatctcct caagatcatc aaggacaagg 4621 acttcctcga caacgaggag aacgaggaca tcctcgagga catcgtcctc actcttactc 4681 tcttcgagga tagggagatg atcgaggaga ggctcaagac ttacgctcat ctcttcgatg 4741 acaaggttat gaagcagctc aagcgtcgcc gttacaccgg ttggggtagg ctctcccgca 4801 agctcatcaa cggtatcagg gataagcaga gcggcaagac tatcctcgac ttcctcaagt 4861 ctgatggttt cgctaacagg aacttcatgc agctcatcca cgatgactct cttaccttca 4921 aggaggatat tcagaaggct caggtgtccg gtcagggcga ctctctccac gagcacattg 4981 ctaaccttgc tggttcccct gctatcaaga agggcatcct tcagactgtt aaggttgtcg 5041 atgagcttgt caaggttatg ggtcgtcaca agcctgagaa catcgtcatc gagatggctc 5101 gtgagaacca gactacccag aagggtcaga agaactcgag ggagcgcatg aagaggattg 5161 aggagggtat caaggagctt ggttctcaga tccttaagga gcaccctgtc gagaacaccc 5221 agctccagaa cgagaagctc tacctctact acctccagaa cggtagggat atgtacgttg 5281 accaggagct cgacatcaac aggctttctg actacgacgt cgaccacatt gttcctcagt 5341 ctttccttaa ggatgactcc atcgacaaca aggtcctcac gaggtccgac aagaacaggg 5401 gtaagtcgga caacgtccct tccgaggagg ttgtcaagaa gatgaagaac tactggaggc 5461 agcttctcaa cgctaagctc attacccaga ggaagttcga caacctcacg aaggctgaga 5521 ggggtggcct ttccgagctt gacaaggctg gtttcatcaa gaggcagctt gttgagacga 5581 ggcagattac caagcacgtt gctcagatcc tcgattctag gatgaacacc aagtacgacg 5641 agaacgacaa gctcatccgc gaggtcaagg tgatcaccct caagtccaag ctcgtctccg 5701 acttccgcaa ggacttccag ttctacaagg tccgcgagat caacaactac caccacgctc 5761 acgatgctta ccttaacgct gtcgttggta ccgctcttat caagaagtac cctaagcttg 5821 agtccgagtt cgtctacggt gactacaagg tctacgacgt tcgtaagatg atcgccaagt 5881 ccgagcagga gatcggcaag gccaccgcca agtacttctt ctactccaac atcatgaact 5941 tcttcaagac cgagatcacc ctcgccaacg gcgagatccg caagcgccct cttatcgaga 6001 cgaacggtga gactggtgag atcgtttggg acaagggtcg cgacttcgct actgttcgca 6061 aggtcctttc tatgcctcag gttaacatcg tcaagaagac cgaggtccag accggtggct 6121 tctccaagga gtctatcctt ccaaagagaa actcggacaa gctcatcgct aggaagaagg 6181 attgggaccc taagaagtac ggtggtttcg actcccctac tgtcgcctac tccgtcctcg 6241 tggtcgccaa ggtggagaag ggtaagtcga agaagctcaa gtccgtcaag gagctcctcg 6301 gcatcaccat catggagcgc tcctccttcg agaagaaccc gatcgacttc ctcgaggcca 6361 agggctacaa ggaggtcaag aaggacctca tcatcaagct ccccaagtac tctcttttcg 6421 agctcgagaa cggtcgtaag aggatgctgg cttccgctgg tgagctccag aagggtaacg 6481 agcttgctct tccttccaag tacgtgaact tcctctacct cgcctcccac tacgagaagc 6541 tcaagggttc ccctgaggat aacgagcaga agcagctctt cgtggagcag cacaagcact 6601 acctcgacga gatcatcgag cagatctccg agttctccaa gcgcgtcatc ctcgctgacg 6661 ctaacctcga caaggtcctc tccgcctaca acaagcaccg cgacaagccc atccgcgagc 6721 aggccgagaa catcatccac ctcttcacgc tcacgaacct cggcgcccct gctgctttca 6781 agtacttcga caccaccatc gacaggaagc gttacacgtc caccaaggag gttctcgacg 6841 ctactctcat ccaccagtcc atcaccggtc tttacgagac tcgtatcgac ctttcccagc 6901 ttggtggtga taagcgtcct gctgccacca aaaaggccgg acaggctaag aaaaagaagt 6961 aggatcctcc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg 7021 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta 7081 acatgtaatg catgacgtta tttatgaggt gggtttttat gattagagtc ccgcaattat 7141 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg 7201 cggtgtcatc tatgttacta gagaattcgg cgcgccgggc ccaacatggt ggagcacgac 7261 actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag 7321 acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt 7381 cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat 7441 aaaggaaagg ctatcgttca agatgcctct gccgacagtg gtcccaaaga tggaccccca 7501 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 7561 tgatgtgata acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca 7621 gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc 7681 ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt 7741 ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc 7801 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt 7861 ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac 7921 gcacaatccc actatccttc gcaagacctt cctctatata aggaagttca tttcatttgg 7981 agaggacacg ctgaaatcac cagtctctct ctacaaatct atctctctcg agatggcggc 8041 caatgcgggc ggcggtggag cgggaggagg cagcggcagc ggcagcgtgg ctgcgccggc 8101 ggtgtgccgc cccagcggct cgcggtggac gccgacgccg gagcagatca ggatgctgaa 8161 ggagctctac tacggctgcg gcatccggtc gcccagctcg gagcagatcc agcgcatcac 8221 cgccatgctg cggcagcacg gcaagatcga gggcaagaac gtcttctact ggttccagaa 8281 ccacaaggcc cgcgagcgcc agaagcgccg cctcaccagc ctcgacgtca acgtgcccgc 8341 cgccggcgcg gccgacgcca ccaccagcca actcggcgtc ctctcgctgt cgtcgccgcc 8401 gccttcaggc gcggcgcctc cctcgcccac cctcggcttc tacgccgccg gcaatggcgg 8461 cggatcggct gtgctgctgg acacgagttc cgactggggc agcagcggcg ctgctatggc 8521 caccgagaca tgcttcctgc aggactacat gggcgtgacg gacacgggca gctcgtcgca 8581 gtggccacgc ttctcgtcgt cggacacgat aatggcggcg gccgcggcgc gggcggcgac 8641 gacgcgggcg cccgagacgc tccctctctt cccgacctgc ggcgacgacg gcggcagcgg 8701 tagcagcagc tacttgccgt tctggggtgc cgcgtccaca actgccggcg ccacttcttc 8761 cgttgcgatc caacagcaac accagctgca ggagcagtac agcttttaca gcaacagcaa 8821 cagcacccag ctggccggca ccggcaacca agacgtatcg gcaacagcag cagcagccgc 8881 cgccctggag ctgagcctca gctcatggtg ctccccttac cctgctgcag ggagtatggg 8941 aagcggagct actaacttca gcctgctgaa gcaggctgga gacgtggagg agaaccctgg 9001 acctatggat ctgcgtctaa ttttcggtcc aacttgcaca ggaaagacgt cgaccgcgat 9061 acgtcttgcc cagcagactg gccttccagt cctttcgctc gatcgggtcc aatgctgtcc 9121 tcaactgtca accggaagcg gacgaccaac agtggaagaa ctgaaaggaa cgacccgtct 9181 ataccttgaa gatcggcctc tggtgaaggg tatcatcgca gccaagcaag ctcacgaaag 9241 gctgatcggg gaagtgtaca attatgaggc ccacggcggg cttattcttg agggaggatc 9301 tatctcgttg ctcaggtgca tggcgcaaag cagttattgg agtaccgatt ttcgttggca 9361 tattattcgc cacaagttag cagacgagga gacattcatg aacgcggcca aggccagagt 9421 taggcagatg ttgcgccctg ctgtaggccc atctattatt caagagttgg ttcatctttg 9481 gaatgagcct cggctgaggc ccatactgaa agagatcgac ggatatcgat atgccatgtt 9541 atttgctagc cagaaccaga tcacacccga tatgctattg cagcttgacc cagatatgga 9601 gggtgagttg attcatggaa tcgctcagga gtatctcatc catgcgcgcc ggcaggagca 9661 ggagttccct ccagtgagcg tggtcgcttt cgaaggattc gaaggtccac cgttcggaat 9721 gtgcggaagc ggagctacta acttcagcct gctgaagcag gctggagacg tggaggagaa 9781 ccctggacct atgaagaaca ataacaacaa atcttcttct tcttctagct atgattcttc 9841 tttgtctcct tcttcttcat cctcctccca ccagaactgg ctctctttct ctctctccaa 9901 caataacaac aacttcaatt cttcctcaaa ccctaatctc acttcctcca catcagatca 9961 tcatcatcct cacccttctc acctctctct ctttcaagct ttctccactt ctccagtcga 10021 acggcaagat gggtcaccgg gagtttcacc cagcgatgcc acggcggttc tttccgtata 10081 ccccggcggt cctaaacttg agaacttcct cggcggagga gcctcaacga cgacaacaag 10141 accaatgcaa caagtgcaat ctcttggcgg cgttgtcttc tcttccgacc tacagccacc 10201 gcttcatcct ccgtccgccg ccgagatcta cgactctgag ctcaagtcaa tagccgctag 10261 cttcctagga aactactccg gtggacactc gtcggaggtc tctagcgtac ataaacaaca 10321 accgaatcct ctagctgtct cagaggcttc gcctactccg aagaagaacg tagagagttt 10381 tggacaacgt acctcgattt atagaggagt cacaagacat agatggactg gaagatacga 10441 agctcatcta tgggataata gttgccgaag agaaggccaa agcagaaaag gaagacaagt 10501 ttatttaggt ggttatgata aggaagataa agcagctaga gcttacgacc ttgcagctct 10561 taagtattgg ggtcctacaa ctacgactaa tttcccgata tcaaattacg aatctgaact 10621 tgaagaaatg aaacacatga ctcgacaaga gttcgttgct tctttaagac ggaaaagcag 10681 tggattctct aggggtgcct ccatgtacag aggcgtcact agacatcatc agcatggtcg 10741 atggcaggca cgaattggaa gagttgcagg caacaaagac ctttatcttg gcacatttag 10801 cactcaagag gaagctgcag aagcttatga tatagcagcg atcaaattcc gcggtctaaa 10861 tgcagtcacc aatttcgaca tcagtcgata tgatgtcaaa tcaattgcta gctgtaatct 10921 ccctgtgggt ggactaatgc ctaaaccttc tccagcaacc gcagcggctg acaaaaccgt 10981 tgatctttct ccatccgact ctccatctct aaccacaccg tccctcacgt tcaatgtggc 11041 aacaccggtc aatgaccatg gaggaacttt ttaccacact ggtataccaa tcaaaccaga 11101 cccggctgat cattattggt ccaacatctt tggattccag gcaaacccga aagcagaaat 11161 gcgaccatta gcaaactttg ggtcggatct tcataaccct tctcctggtt atgctataat 11221 gccggtaatg caggaaggtg aaaacaactt tggtggtagt tttgttgggt ctgatgggta 11281 taacaatcat tccgctgcat cgaacccggt ctcagcaatt ccgctgtcct cgacaactac 11341 aatgagtaac ggtaacgaag ggtatggtgg aaacataaac tggattaata acaacatttc 11401 aagttcttac caaactgcaa aatcaaatct ctctgttttg cacacaccgg tttttgggtt 11461 ggaatagctc gagtttctcc ataataatgt gtgagtagtt cccagataag ggaattaggg 11521 ttcctatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat 11581 ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc agtactaaaa 11641 tccagatccc ccgaattaat tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt 11701 tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc 11761 aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag 11821 tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga 11881 tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag 11941 ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc gcggtttcaa 12001 aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg aaaaagctgt 12061 tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg tcttgttata 12121 attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata ataaatggct 12181 aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat 12241 acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat 12301 ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac 12361 atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat 12421 gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat 12481 gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt 12541 cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa 12601 ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaagac 12661 actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag 12721 gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa 12781 gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc 12841 ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga gctatttttt 12901 gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa 12961 ttgttttagt acctagaatg catgaccaaa atcccttaac gtgagttttc gttccactga 13021 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 13081 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 13141 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 13201 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 13261 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 13321 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 13381 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 13441 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 13501 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 13561 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 13621 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 13681 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac 13741 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc 13801 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg 13861 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag 13921 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc 13981 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac 14041 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac 14101 gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt 14161 ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc agcggccgcg 14221 ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt aggcgctttt 14281 tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg ggttttaaga 14341 gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc ttttatatca 14401 gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat gtacgggttc 14461 cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg aaagagacct 14521 tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt acattaggaa 14581 ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat cgggccagcc 14641 tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc 14701 acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta gatcgtcttg 14761 aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg 14821 ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg 14881 ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta ctccggccgc 14941 ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc 15001 accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac 15061 cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg ccggcagaac 15121 ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc cttcccttcc 15181 cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc 15241 cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg 15301 tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc 15361 atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa aaaatctggt 15421 tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg cggttgccgg 15481 gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg tgcttctgcc 15541 tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag gtcatcaccc 15601 agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgtcacgccg attcctcggg 15661 cttgggggtt ccagtgccat tgcagggccg gcagacaacc cagccgctta cgcctggcca 15721 accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt gttcttgatt 15781 ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat ttgctcattt 15841 actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg ccttggcgta 15901 ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca 15961 tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac 16021 ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat taactcaaat 16081 gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt cgccctcggg 16141 ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt 16201 gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc 16261 gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt 16321 gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata tgtcgtaagg 16381 gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca cagccaagtc 16441 cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc 16501 gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt cccgcacggc 16561 cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat cggccccggc 16621 gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg 16681 gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt tactcatcgc 16741 atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca tcaccttttt 16801 agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc cagcttggca 16861 tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg 16921 aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa aaacggttcg 16981 tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc tcggcggccg 17041 ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg 17101 tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa 17161 gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg 17221 cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg gccttggcgg 17281 cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg 17341 cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac ggctctgcca 17401 ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg 17461 tgctgcgggc caggcggtct agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt 17521 caagcatcct ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca 17581 gcttggtgca gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg 17641 tgctgacgcg ggcatagccc agcaggccag cggcggcgct cttgttcatg gcgtaatgtc 17701 tccggttcta gtcgcaagta ttctacttta tgcgactaaa acacgcgaca agaaaacgcc 17761 aggaaaaggg cagggcggca gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt 17821 cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg aggggttgga 17881 tcaaagtact ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa 17941 cggataaacc ttttcacgcc cttttaaata tccgttattc taataaacgc tcttttctct 18001 taggtttacc cgccaatata tcctgtcaaa cactgatagt ttaattcccg atctagtaac 18061 atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt gttttctatc 18121 gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat aaataacgtc 18181 atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta tatgataatc 18241 atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat gtttgaacga 18301 tcggggaaat tcgagctggt cacctcacta tcactggagg cttggctcaa gtttggcctg 18361 catgacggcg tcgaggaaat cgtcgatcgc cttcacggag gagcccttca gctctttttc 18421 ctcgttcacg gcgtccttga gctttttgcc catttccacg gcgcgattct tcatctccca 18481 gcctttggag ccctcgttgc gctcaaggat catctcgacg atgcgcttga ccttctcttt 18541 ggagatctcg ccttcgaggc ctctagcaag ctcaaccgcg acgcccatct catcttcgag 18601 gtacttgagg ttgtaggcct gctcggcagc aagtggccat ccaagcatcg gaacgccctc 18661 gcgaagagac tcaaggatgc tgttccatcc gcagtgggtg agaaagccgc cgatggattc 18721 atggttgagg atctcaagct gcgggccgag cttgtacacg agcttgccct gtttcttcac 18781 cttcatgcgt tcctcaaagc cctctggcag ccactctggg cgcatctctt cgttgatgtc 18841 gaagccgaac ggggctctga tcacccagag gaatggcttc tcggaggact caaggccagc 18901 agcgagttcc atcatctgcg tcgggctgat cgtgttctgg gagccgaagg agatgtagag 18961 gacggagtcc ggctctttga ggctgagcca ctgcacgaac tcggcgcctg ttgaattgtt 19021 gtcggaggag gagtgctgca ctggggaagc aataagtggg ccgatgcccc agatcggcag 19081 cttggtgtag ttgcggagga tggagaagcc gagtgtctcg atctcttcca cggagttgca 19141 gagccagcca aaagacttca tggactggcg gagctgcggc tggaaatact tggaccaatc 19201 gtcggagccg tcggcatatc tgaggaaccg atggagctgg gacctgcgga acttatggtt 19261 ctctgggaag cctggcagcg ggaactcttg gtcgtcggag tagttctggt gcgggagatc 19321 gttccagatg gacacgtagg cggatgtgcc ataagcgccg cctgttgtga acaccacgcc 19381 tgtagagccc acatccttgg ccacctggtc aacccagccg aggaacacat catggatcac 19441 gcagagtggt ggcctggctg ggctgaaatg cctggagatg taatcgcgga ggtgcggatc 19501 gaggctgatg gtggaatgga agagggacac cacgagcggg agtgtgagct tatcggtgtt 19561 ctcgatgccc ggtgggagtc cgtggttggt ggagttgaat ggcagctcga cgatcctgat 19621 gccgctggag gagtaggaat ggtggtgaag gaggtgcctg aggaagccgg cgttgagcgg 19681 agttgtgagc agggtgatga tcacgtggga gcgcttgtag aggaacatgg cgagttcgag 19741 aaatggcctg aggtggccct gcgccatgaa cgggatcatg aggatgtgct gtgtctcgcc 19801 ctcgccgttg gtgttcatct tgatggcggt aggtccagga ttagattcaa catctccagc 19861 caacttcaac aaatcaaaat tcaacaattg ggcggaggtg aacttgtagg agccgtggca 19921 aagtgtgcca tgatcccagg aggagtggat cagctcggcc ttccactttt caccagcagc 19981 gccgagcaca acgtggagcg ggtagaagtg ctctggaaat ggatgggcga gcttccagtt 20041 cggggccttg ctctcgtaga tgttcacttc ctcgaagcgg ccgttgatga gggccttcct 20101 aagccaagaa tcgaaggcag cggcccaagg ggcaacacca tcgaagtagt gcggtgtctc 20161 atcgagtgga tgtgtggcgc tgccggagcc aataatgagc acgccatcat tcttgagcgg 20221 cgcgagggct ctgccgagat tgtagtggta ggtgccgtcg agatgtggct gaactgagag 20281 ctggcacacc gggatgtccg cctctggata catgagcatg agtggaaccc aggcgccgtg 20341 atcaaggccc ctcttttggt ctgtctcggc tgtctcgaac ccggacttct tgaggatctc 20401 ttccaccttt ctcgcaagct ctggctcgcc tggagctggg tacttgaact ggtacatggc 20461 ggctgggtag tcgtcgaagt cgtggatggt gtcgttgatg tgcacggcgt tcacggttgg 20521 cttcactgtc tcccagtggc cggagatgat gaggatggcc ttcggcttct tggagaaaat 20581 cttctcgcgc catgtctcga agaacggcct gagcggatgt gtatcctcga cggtgaggat 20641 cgggttgccg tgggtgatga agaaggactc tttgatcatc tggtcgttgg cgtcctcgcc 20701 gttcatcatc ttaggtccag gattagattc aacatctcca gccaacttca acaaatcaaa 20761 attcaacaat tggtagcgcg gaatcgggat gagcttgagc ggcttggtct tttggagcgc 20821 aatgccgaac ttctcgtcca tgtcgaggtc cttcggggag atgtcgcctt cgagcttcca 20881 gttgaagaac tggaggagtg tggcgagcat gagggtgagc atcctgatgg cgagattcat 20941 gcctgggcaa attctgcggc cagcgccaaa tggaaggagg ccgaaatcgc ggcccttaac 21001 atcgatctcg cagccgatga agcgctctgg gctgaaaata tcggcgttct gccaggcgtt 21061 tgggtccctg ccaatggccc agaggttcac gaggatctgg gcatccttcg gcacgatgta 21121 gccgtacagc tcgacatcgg tgtcggcctt tcttgggagc aagaacacgg tcggcggatg 21181 gaggcggagt gtctctttga tgatcgcctg gaggtacggc aggttgatga tgtcggactc 21241 ttggatctgc ttgtccttgc cgaggacctg cttgatttcc tcttgggcct tttccatcat 21301 ctctggattg cggatcagct cggtcatcac ccactcgaat gtggaggatg tggtatcggt 21361 gccggcgtcg aagatgtcca cgaggaggtg gttgatctcg cccatcgtca gctcgttctg 21421 cttgaacagc tggaggagca catcgagcac gtcgtcggtg gtggtggtag ttgtggtgga 21481 ggaatctggg gcgagtctct cgcagatgat gccctggaac acggcgatca gcttgtcgaa 21541 ggagcaggcg agccttcttc taatgccaga tgggtcaacg cagccgagaa tcgggaagta 21601 gtcggcgtag ttcggcttgc ctatatcttc catgatgttc cagatcagct ctttgaactc 21661 ttggctggta tgggacttgt ggtgcgccag ctcgacgctg aagaacagct tgctgaggag 21721 gttgaggctg gtcgtaaagg cggccttgcc aatatccacg gcctggcctt tctgggcgca 21781 ctcttgaacg tactcgtaga gctgctgcac cttggcgtgc ctgaatgtct ggcaggcatc 21841 gagtctctgt ggggagagca gatgcacggc ggtaatcttg cggaagttgc gccacttcgg 21901 agacaccggg agccaagaca tggtgagctt gtggtggtcg ccggctgtaa cactattcgg 21961 gatcgtgcgg ttggagagcg ggtgatcctt cttgaggaac atctctttgg ccacgtcggc 22021 agaggacaca acgatggtgg tcacagagcc gagtctgagg gaaatgagtg ggccgtgaat 22081 cttggcgagg ttggcgaagg accgatgcgg cttcttgccc acctcaagga tgttgccgat 22141 gatcggaagc ggctttggtc ctggcggaag cagcttggtg gtctgctggg agaacagcag 22201 cttgatgaag tggaagctga tgaaccagat cgcgaggatc atggcgaggg tcgcatgat //